ABSTRACT The objective of the present study was to evaluate the stability of candidate reference genes and select the genes that can be used for normalizing real-time polymerase chain reaction (PCR) in the liver, skeletal muscle, and jejunum tissues of Nellore or Nellore × Angus steers fed different diets. Fourteen purebred and 14 crossbred steers were used, in which half of the animals of each genetic group received a diet containing whole shelled corn (WSC) and the other half whole shelled corn and sugarcane bagasse (WSCB). Stability was analyzed by the RefFinder program. To validate the selection of candidate reference genes, the expression of target genes was evaluated using the different groups of reference genes. The most stable genes were 18S, ACTB, and CASC3 for skeletal muscle; HMBS, ACTB, and 18S for the liver; and GAPDH, ACTB, and CASC3 for the jejunum, regardless of breed and diet provided. Possible errors caused in data analyses were clarified comparing the more and less stable genes as reference for normalization of the target genes FASN, ACOX, SCD1, MGAM, and SLC2A1. The use of the more stable and less stable sets of reference genes may lead to different conclusions in respect to the expression profile of the target studied gene. The selection of more suitable reference genes for each experiment is of utmost importance to ensure the reliability of gene expression studies so that they can be applied in practice.

O objetivo deste trabalho foi avaliar o desempenho, as características de carcaça e as suas correlações com as expressões gênicas em tourinhos. Quarenta animais Red Norte foram confinados e distribuídos em delineamento inteiramente casualizado, em arranjo fatorial 2x2, e alimentados com grãos de soja moídos ou gordura protegida, com ou sem inclusão de monensina. As dietas foram formuladas para serem isonitrogenadas, com teores de extrato etéreo semelhante (6,5%), e tiveram silagem de milho como volumoso (40%). O período experimental foi de 84 dias, precedido por 14 dias de adaptação. Foram avaliadas as expressões dos genes PPARA, SREBP1c e SCD1. Animais alimentados com gordura protegida tiveram maior ganho de peso nos primeiros 56 dias de confinamento. O uso da monensina não afetou o ganho de peso diário dos animais, mas proporcionou aumento do rendimento de carcaça. Houve correlação de -0,40 entre o ganho de peso dos animais e a expressão do SCD1. O uso de gordura protegida aumenta o desempenho dos animais no início do confinamento, e a monensina proporciona maior rendimento de carcaça.

The objective of this work was to evaluate performance, carcass traits, and their correlation with gene expression in young bulls. Forty Red Norte animals were feedlot and distributed in a completely randomized design, using a 2x2 factorial arrangement, and fed ground soybean grains or rumen-protected fat, with or without monensin supplementation. Diets were formulated to be isonitrogenous, with similar ether extract contents (6.5%), and had corn silage as roughage (40%). The experimental period lasted 84 days, preceded by 14 days of adaptation. The expressions of the PPARA, SREBP1c and SCD1 genes were evaluated. Animals fed rumen-protected fat had greater average daily gain in the first 56 days of feedlot. Monensin did not affect the daily gain, but increased carcass weight. There was -0.40 correlation between animal daily gain and SCD1 expression. The use of rumen-protected fat increases animal performance at the beginning of feedlot, and monensin provides greater carcass weight.

Este trabalho foi realizado com o objetivo de avaliar as características da carcaça em novilhos Zebuínos alimentados com inclusão de fontes de lipídeos moídas. Utilizaram-se 31 novilhos Zebuínos com idade média inicial de 23 meses e peso vivo inicial de 365 + 37,5 kg. As dietas consistiram em silagem de milho e quatro concentrados, sendo um sem lipídeo adicional (SLA) e três com a inclusão de diferentes grãos de oleaginosas moídas: grão de soja (GS), caroço de algodão (CA) e semente de linhaça (SL). As rações apresentaram relação concentrado:volumoso 60:40 e foram fornecidas ad libitum. O período experimental teve duração de 84 dias sendo precedido de um período de adaptação de 28 dias. O delineamento utilizado foi em blocos casualizados, sendo as médias dos tratamentos comparadas pelo teste Scott & Knott no nível de 5% de probabilidade. Com a inclusão de CA observou-se diminuição (p < 0,05) do rendimento de carcaça dos animais. O traseiro especial foi mais pesado (p < 0,05) com a inclusão GS na dieta. O maior rendimento (p < 0,05) da ponta de agulha e maior peso (p < 0,05) de coxão duro foram verificados com a inclusão de CA e SL na dieta. A inclusão de GS na dieta melhora as características de carcaça.

This research aimed to evaluate carcass traits of Zebu steers fed different oleaginous grains. Thirty one 23 months old zebu steers with 365 + 37.5 kg of live weight were used. The diets consisted ofcorn silage and four different concentrates; no additional lipids and three different ground oilseeds sources: soybean, cottonseed and linseed. The diets had concentrate:roughage ratio of 60:40 and were offered ad libitum. The experimental period was of 84 days, preceded by a 28 day adaptation period. The experiment was carried out in a randomized block design and the means were compared by Scott-Knott test at 5% level of probability. The CS inclusion of into the diet decreased (p < 0.05) carcass yield of the animals. The hindquarter was heavier (p < 0.05) with the addition of SB to the diet. The spare ribs had the highest performance (p < 0.05) and outside flat was heavier (p < 0.05) when the animals were submitted to CS and FS diets. The CS addition to the diet improved carcass characteristics.

Objetivou-se com este trabalho avaliar as características de carcaça e cortes comerciais de tourinhos Red Norte suplementados com óleos de fritura e de soja terminados em confinamento. Utilizaram-se 27 animais Red Norte com peso corporal inicial médio de 317 ± 33kg. Os animais receberam ração ad libitum com relação volumoso:concentrado 40:60, sendo: dieta controle; suplementados com óleo de soja e suplementados com óleo de fritura, durante 112 dias, sendo 28 dias de adaptação, e abatidos com 481,0; 488,7 e 462,6kg para os tratamentos controle, suplementados com óleo de soja e óleo de fritura respectivamente. A espessura de gordura subcutânea (EGS) e a área de olho de lombo (AOL) foram medidas entre a 12ª e 13ª costelas. A suplementação com fontes lipídicas não influenciaram o peso final, peso de carcaça quente, peso de carcaça fria, AOL, EGS, perdas por resfriamento, pesos e rendimentos dos cortes dianteiro, traseiro especial e ponta de agulha. Entretanto o rendimento de carcaça foi menor para animais suplementados com óleo de soja. O pH final foi maior (P<0,01) para as carcaças dos animais suplementados com óleo de fritura, e a temperatura final (P<0,02), foi maior para os animais com suplementação lipídica em relação aos animais sem suplementação. A suplementação lipídica utilizada para tourinhos em confinamento não influenciou as características de carcaça. Entretanto, animais suplementados com óleo de fritura apresentaram carne DFD, enquanto os animais suplementados com óleo de soja apresentaram menor rendimento de carcaça.

The objective of this study was to evaluate the characteristic of carcass and commercial meat cuts from Red Norte young bulls finished in feedlot supplemented with fry and soybeans oil. Twenty seven Red Norte animals at initial average weight of 317 ± 33kg were used. The animals received ad libitum feed with concentrate:roughage ratio of 40:60, being: control diet; supplementation with soybeans oil and supplementation with fry oil, during 112 days, from which 28 days were for adaptation, and slaughtered with 481,0; 488,7 e 462,6kg to treatments control, supplemented with soybeans oil and with fry oil respectively. The subcutaneous fat thickness (FT) and longissimus muscle area (LMA) were measured between the 12th e 13th ribs. The supplementation with lipids sources did not influence the final weight, hot carcass weight, cold carcass weight, LMA, FT, losses by cooling, weight and yield of the forequarter, hindquarter and spare ribs cuts. However the carcass dressing was lower to animals supplemented with soybeans oil. The final pH was higher (P<0,01) to carcasses of the animals supplemented with fry oil, and the final temperature (P<0,02) was higher to animals supplemented with lipids than to animals without supplementation. The lipid supplementation used to young bulls in feedlot did not influence the carcass characteristics. However, animals supplemented with fry oil presented DFD meat, whereas animals supplemented with soybeans oil had lower dressing carcass.

The objective of this research was to evaluate average daily gain (ADG), carcass traits, meat tenderness and profitability of keeping cattle fed different oilseeds and vitamin E in feedlot. A total of 40 Red Norte young bulls with initial average body weight of 339±15 kg were utilized. The experimental design was completely randomized in a 2 × 2 factorial arrangement. The experiment lasted 84 days and experimental diets presented soybeans or cottonseeds as lipid sources associated or not to daily supplementation of 2,500 UI vitamin E per animal. The concentrate:roughage ratio was 60:40. Diets had the same amount of nitrogen (13% CP) and ether extract (6.5%). The data were analyzed by means of statistical software SAS 9.1. Neither vitamin supplementation nor lipid source affected ADG. There was no interaction between lipid source and vitamin supplementation for the variables studied. The inclusion of cottonseed reduced the carcass yield. There was no effect of diets on hot and cold carcass weights or prime cuts. The inclusion of cottonseed reduced the backfat thickness. No effect of experimental diets on the rib-eye area was observed. There was no effect of lipid source or vitamin supplementation on meat tenderness, which was affected, however, by ageing time. Diets with soybeans presented higher cost per animal. The utilization of soybean implied reduction of the gross margin (R$ 59.17 and R$ 60.51 for diets based on soy with and without supplemental vitamin, respectively, vs. R$ 176.42 and R$ 131.79 for diets based on cottonseed). The utilization of cottonseed enables improvement of profitability of feedlot fattening, in spite of negatively affecting some carcass characteristics.

The objective of this study was to evaluate the residual feed intake (RFI) and its relation with performance, carcass and meat characteristics of Zebu animals. A total of 40 Zebu steers with initial age of 18 months and initial live weight of 350 kg were confined in feedlot for 112 days. The average daily gain (ADG) and dry matter intake (DMI) were obtained every 14 days. Residual feed intake was determined by the difference between observed and predicted DMI, based on the metabolic weight and the ADG. Animals were classified into the RFI groups: high (>0.5 standard deviation of the mean - least efficient), medium (±0.5 standard deviation of the mean) and low (<0.5 standard deviation of the mean - most efficient). Daily cost with feeding and per kilogram of weight gain was determined. Characteristics of carcass (weight, yield, subcutaneous fat) meat (pH, shear force and color) were assessed. Animals with low RFI presented lower DMI. The variation in DMI between the low and the high RFI groups promoted difference in the daily cost with feeding and per kilogram of weight gain, with animals of low RFI presenting costs 5 and 9% lower, respectively. Carcass and meat characteristics differed over the RFI groups. The knowledge of the RFI of the animals enables a more efficient system, with reduction in the costs with feeding, without promoting alterations on the performance and carcass characteristics of the meat from Zebu animals.

Objetivou-se com este trabalho avaliar as características de carcaça e o peso dos cortes comerciais da carcaça de tourinhos Red Norte e Nelore terminados em confinamento. Utilizaram-se 44 animais, sendo 22 Red Norte com peso vivo médio inicial de 367±30 kg e 22 Nelore com peso vivo médio inicial de 361±30 kg. Os animais receberam ração à vontade com relação concentrado:volumoso de 50:50 durante 112 dias, sendo 28 dias de adaptação, e foram abatidos aos 519 e 482 kg para Red Norte e Nelore, respectivamente. A espessura de gordura subcutânea (EGS) e a área de olho-de-lombo (AOL) no músculo longissimus dorsi foram medidas entre a 12ªe 13ª costelas. Observou-se maior rendimento de carcaça nos animais Nelore (57,7 vs 54,7%). Entretanto, não houve diferença no peso de carcaça quente e fria entre os grupos. Os animais Red Norte apresentaram maior AOL, maiores pesos e rendimentos de traseiro e ponta-de-agulha, e menor peso e rendimento de dianteiro. O grupo genético Red Norte apresentou maior peso de picanha e contrafilé, enquanto o Nelore apresentou maior peso de paleta e coxão duro. Animais Nelore apresentam maior rendimento de carcaça em relação aos Red Norte, o que favorece o peso de carcaça quente, principal forma de remuneração paga aos produtores. Todavia, animais Red Norte apresentam maior rendimento de traseiro e maior peso dos cortes de maior valor comercial.

The objective of this study was to evaluate the characteristics of carcass and weight of the commercial meat cuts from carcass of Red Norte and Nellore young bulls finished in feedlot. Forty four animals - 22 Red Norte with initial average weight of 367±30 kg and 22 Nellore at initial average weight of 361±30 kg - were used. The animals received feed ad libitum with concentrate:roughage ratio of 50:50 during 112 days, from which 28 days were for adaptation. Red Norte and Nellore animals were slaughtered at 519 and 482 kg, respectively. The subcutaneous fat thickness (FT) and longissimus muscle area (LMA) were measured between 12th and 13th ribs. Higher carcass yield of the Nellore breed was observed (57.7 vs 54.7%). However, there was no difference for weight of hot and cold carcass between the groups. Red Norte animals showed greater LMA, and hindquarter and spare ribs with higher weight and yield, and forequarter with lower weight and yielding. The Red Norte genetic group showed higher weight for cap and rump and striploin, while Nellore showed higher weight for shoulder and flatround. Nellore animals had better carcass yield, which contributes to hot carcass weight. Nevertheless, the group Red Norte have higher hindquarter yield and more weight from cuts of highest commercial value.

Objetivou-se com este trabalho avaliar a composição química e o perfil de ácidos graxos do músculo longissimus dorsi e da gordura subcutânea de tourinhos Red Norte e Nelore terminados em confinamento. Utilizaram-se 44 animais, sendo 22 Red Norte com peso vivo inicial médio de 367±30 kg e 22 do grupo Nelore com peso vivo inicial médio de 361±30 kg. Os animais receberam ração à vontade durante 112 dias e foram abatidos com 519 e 482 kg, respectivamente. Amostras do músculo longissimus dorsi e da gordura subcutânea foram coletadas 24 horas após abate entre a 12ª e 13ª costelas para análise da composição centesimal e do perfil de ácidos graxos. As análises de ácidos graxos foram realizadas por meio de cromatografia gasosa, em coluna capilar de 100 m. Não houve diferença na composição química da carne entre os grupos genéticos. Nos animais Red Norte, foram maiores os teores dos ácidos graxos pentadecanoico, palmítico, palmitoleico, linoleico e ácido linoleico conjugado (CLA), enquanto nos animais Nelore foi encontrado o maior teor de ácido oleico. O músculo longissimus dorsi apresentou maiores teores dos ácidos láurico, heptadecenoico, esteárico, linoleico, α-linolênico e araquidônico. Em comparação ao músculo longissimus dorsi, na gordura subcutânea foram maiores os teores dos ácidos mirístico, miristoleico, pentadecanoico, palmítico, palmitoleico, oleico e CLA. Os animais Red Norte apresentaram maiores teores de ácidos graxos saturados em comparação aos Nelore. Em bovinos, o perfil de ácidos graxos depositados no músculo é diferente do observado na gordura subcutânea. O perfil de ácidos graxos da carne de tourinhos difere entre grupos genéticos.

The objective of this study was to evaluate the chemical composition and the fatty acid profile of the longissimus dorsi muscle and the backfat thickness of Red Norte and Nellore young bulls finished in feedlot. Fourty-four animals (22 Red Norte with initial average weight of 367±30 kg and 22 Nellore at initial average weight of 361±30 kg) were used. The animals were fed ad libitum during 112 days, slaughtered at 519 and 482 kg, respectively. Samples of the longissimus dorsi muscle and backfat thickness were collected 24 hours after slaughter between the 12th and 13th ribs for analysis of the chemical composition and the fatty acids profile. Fatty acid analyses were performed by gas chromatography with a 100-m capillary column. There was no difference for the chemical composition of the meat between the genetic groups. Red Norte animals showed higher contents of pentadecanoic, palmitic, palmitoleic, linoleic and conjugated linoleic acid (CLA) fatty acids, while Nellore animals had the highest content of oleic acid. The muscle longissimus dorsi showed greater concentrations of lauric, heptadecenoic, stearic, linoleic, α-linoleic and arachidonic acids. Comparing the fatty acid deposited in the longissimus dorsi muscle with backfat thickness, the latter showed higher contents of miristic, miristoleic, pentadecanoic, palmitic, palmitoleic, oleic and CLA acids. Red Norte animals showed higher contents of saturated fatty acid than Nellore. In bovine, the profile of fatty acids deposited in the muscle is different than the that in the backfat thickness. The fatty acids profile of the meat from young bulls differs between genetic groups.

The objective of this study was to study the animal reactivity and correlate it with performance and meat quality of cattle finished in feedlots. A total of 20 animals of the Tabapuã breed and 20 animals of the Nellore breed were confined for 112 days: 28 days of adaptation and 84 days of data collection. Performance was determined by the average daily weight gain (ADG) and average dry matter intake (DMI) estimated through the use of indicators. On the 1st, 42nd and 84th days of the experiment, two methods were used to measure the animal reactivity: animal reactivity in mobile cage and visual reactivity during handling. The characteristics of meat quality were evaluated by the color, shear force and pH24 after slaughter (pH24). Through the study of correlation, the degree and direction of linear association on animal reactivity, performance and meat quality were established. Regression equations were generated for the parameters evaluated according to the values of animal reactivity. Correlations between mobile cage and visual reactivity were 0.77, 0.56 and 0.45 in the 1st, 2nd and 3rd evaluations, respectively. There was no correlation between animal reactivity and DMI. The correlations between ADG, pH24, and color with animal reactivity were -0.31, 0.40, 0.47 and -0.34, and -0.33 for mobile cage, 0.74, 0.71 and -0.51, for visual reactivity, respectively. The parameters of meat quality varied according to the reactivity. Undesirable values of pH24, animal reactivity and color were found when mobile cage reactivity was higher than 780, 590 and 540, respectively. In general, animals that show greater reactivity tend to have lower daily weight gain and lower meat quality. The reactivity can be used in animal breeding programs, in order to improve performance and meat quality of Zebu cattle finished in feedlot.

Objetivou-se com este trabalho avaliar os efeitos de dois teores de silagem de milho e da suplementação com soja extrusada sobre o perfil de ácidos graxos na gordura do leite. Oito vacas da raça Holandesa foram distribuídas em um quadrado latino duplo 4 x 4, em arranjo fatorial. Os tratamentos foram: alta silagem de milho e baixo lipídio, alta silagem de milho e alto lipídio, baixa silagem de milho e baixo lipídio e baixa silagem de milho e alto lipídio. Os períodos experimentais tiveram duração de 21 dias cada. As amostras de leite foram coletadas nos dias 18 e 19 de cada período experimental. O aumento dos teores de lipídios nos dois níveis de silagem diminuiu as concentrações de ácidos graxos de cadeia curta (C4:0 - C12:0). O teor de ácido linoleico conjugado (cis-9 trans-11 C18:2) aumentou de 3,72 para 4,85, e de 4,60 para 5,89mg/g, quando a soja extrusada foi utilizada em dietas com alto e baixo nível de silagem de milho, respectivamente. O aumento no teor de lipídios na dieta resultou em maior concentração de trans-11 C18:1. O ácido graxo trans-10 C18:1 tendeu a aumentar com a elevação do teor de lipídios na dieta. Houve correlação significativa entre trans-10, cis-12 C18:2 e percentual de gordura do leite. A suplementação com soja extrusada diminuiu os ácidos graxos de cadeia curta e o ácido palmítico no leite. Além disso, esta oleaginosa aumentou a concentração de ácido linoleico conjugado (C18:2 cis-9 trans-11) e vacênico, com o aumento da qualidade do leite para o consumo humano.

The objective of this study was to evaluate the effects of two levels of maize silage and extruded soybeans supplementation on fatty acid profile in milk fat of dairy cows. Eight Holstein cows were distributed in a double 4x4 Latin Square design with a factorial arrangement. The treatment groups were: high maize silage and low lipid, high maize silage and high lipid, low maize silage and low lipid and low maize silage and high lipid. The experimental periods lasted 21 days. Milk samples were collected on days 18 and 19 of each experimental period. The increase of lipid contents in high and low maize silage diets decreased short chain fatty acids concentrations (C4:0 - C12:0). The conjugated linoleic acid content (cis-9 trans-11 C18:2) increased from 3.72 to 4.85, and 4.6 to 5.89mg/g, when extruded soybeans was used in high and low level of corn silage, respectively. The increase in dietary lipids content resulted in higher trans-11 C18:1 fatty acid concentration. Fatty acid trans-10 C18:1 tended to increase with the elevation of the lipids content in the diet. There was significant correlation between trans-10, cis-12 C18:2 and milk fat percentage. The extruded soybeans supplementation decreased shortchain fatty acids and palmitic acid in milk. In addition, this oilseed enhanced the concentration of conjugated linoleic acid (C18:2 cis-9 trans-11) and vaccenic acid, which improved the milk quality for the human health.

Avaliaram-se o consumo, o desempenho e a digestibilidade de dietas contendo diferentes grãos de oleaginosas em novilhos zebuínos em confinamento. Utilizaram-se 30 novilhos zebuínos com peso vivo inicial de 388,0±37,5 kg, distribuídos em delineamento inteiramente casualizado. As dietas foram formuladas com silagem de milho e quatro concentrados: um sem suplementação de lipídeos e três com a inclusão de grão de soja, caroço de algodão ou semente de linhaça. As rações, isonitrogenadas, apresentaram relação concentrado:volumoso 60:40 e foram fornecidas à vontade. Para determinação da digestibilidade aparente total, a produção fecal foi estimada utilizando-se fibra em detergente neutro indigestivel como indicador interno. O menor consumo de matéria seca (7,20 kg/dia) foi observado para a dieta contendo caroço de algodão, mas, entre as outras dietas, não houve diferenças nesta variável. O ganho médio diário foi menor quando se utilizou a dieta contendo caroço de algodão em relação à dieta sem lipídeo (0,93 vs. 1,16 kg/dia, respectivamente). No entanto, não houve diferença no ganho médio diário destas dietas em relação às demais. Houve redução da digestiblidade da fibra em detergente neutro na dieta contendo semente de linhaça. Adicionalmente, houve redução na digestibilidade dos carboidratos não-fibrosos nas dietas contendo caroço de algodão e semente de linhaça em relação à dieta com grão de soja, a qual não diferiu da dieta controle. A inclusão de caroço de algodão moído em dietas para bovinos em confinamento deve ser feita com cautela, pois pode provocar redução no consumo de matéria seca e no ganho médio diário. No entanto, a eficiência alimentar não é afetada pela adição de oleaginosas na dieta de bovinos de corte quando o nível máximo de extrato etéreo na matéria seca total da dieta não excede 6%.

This study was conducted to evaluate feed intake, performance and of digestibility diet containing different oilseeds to beef cattle finished in feedlot. Thirty zebu steers (388.0±37.5 kg of body weight) were used in a completed randomized design. The diets consisted of corn silage and four different concentrates: one without lipids and three with inclusion of soybeans, cottonseed and linseed. The diets, which were isonitrogenous, had roughage:concentrate ratio of 60:40 and were offered ad libitum. To determinate the total apparent digestibility of nutrients, the fecal output was estimated through internal marker indigestible neutral detergent fiber. The lowest dry matter intake was observed for cottonseed (7.20 kg/d), but among the other diets, the dry matter intake did not differ. The average daily gain was lower when cottonseed was used, in comparison with linseed (0.93 vs. 1.16 kg/d, respectively). However, there was no difference on average daily gain in relation to other diets. There was reduction in the digestibility of neutral detergent fiber in linseed diet. Additionally, there was a reduction in the neutral detergent fiber digestibility in diets with cottonseed and linseed compared with soybeans, which did not differ from the control diet. The inclusion of ground cottonseed in feedlot beef cattle diets should be realized with caution, since it can decrease dry matter intake and average daily gain. However, feed efficiency is not affected by the addition of oilseeds in beef cattle diets when the maximum ether extract level in total dry matter diet does not exceed 6%.

Conduziu-se este trabalho, com o objetivo de verificar a viabilidade econômica da terminação em confinamento de novilhos Nelore e Red Norte na região de Lavras, MG. Foram utilizados 41 novilhos, não castrados, de dois grupos genéticos, sendo: 19 Nelore e 22 Red Norte, com pesos vivos iniciais médios de 315 ±31 kg e 321 ±30 kg, respectivamente. O período experimental foi de 112 dias, divididos em quatro períodos de 28 dias. A viabilidade econômica foi analisada utilizando o modelo de custo, considerando os custos operacionais, alternativos e de depreciação. Todos os indicadores econômicos gerados apresentaram resultados negativos. A atividade apresentou prejuízo de R$ 6,88 e 19,74/arroba para os animais Red Norte e Nelore, respectivamente. Os resultados negativos obtidos nessas condições ocorreram porque o custo operacional efetivo foi maior que o preço de venda da arroba. Em um cenário com a aquisição de animais a atividade de confinamento apresentou resíduo próximo de zero para o grupo Red Norte. A prática do confinamento não apresentou viabilidade econômica para a região de Lavras, MG, no ano de 2007.

The objective was to study the economic viability of feedlot in Lavras region, state of Minas Gerais. Forty one bulls of two genetic groups were used, being: 19 Nellore and 22 Red Norte, with initial average age of 20 months and initial average live weight of 315 ±31 kg and 321 ±30 kg, respectively. The experimental period was 112 days. The model of cost was used to evaluate the economic viability. All economic indexes presented negative results. The activity presented negative profit of R$ 6.88 and R$ 19.74/arroba for the animals Red Norte and Nellore, respectively. The negative results obtained in these conditions took place because the operational effective cost was bigger than the sale price of the arroba. The feedlot didn't present economical viability for the Lavras region, in the year of 2007. The feed efficiency has straight influence on the economical viability of feedlot.

Objetivou-se avaliar o ganho médio diário (GMD) e as características de carcaça em novilhos das raças Nelore e Red Norte, não-castrados, terminados em confinamento e avaliar as predições do desempenho pelos sistemas CNCPS 5.0, NRC (2000) e BR-CORTE. Utilizaram-se 41 novilhos: 19 do grupo Nelore com peso vivo inicial de 361 ± 31 kg e 22 do grupo Red Norte com peso vivo inicial de 367 ± 30 kg. O período de adaptação teve duração de 28 dias. A avaliação do desempenho animal foi composta de três períodos de 28 dias, totalizando 84 dias. Ao final de cada período, realizou-se a pesagem dos animais após jejum alimentar de 16 horas. Na raça Red Norte, o ganho médio diário foi superior ao da raça Nelore (1,81 vs. 1,43 kg/dia). Os animais da raça Red Norte apresentaram também maior área de olho-de-lombo (75,41 cm² vs. 68,67 cm²). Não foi observada diferença nas espessuras de gordura subcutânea e de gordura na garupa entre os grupos genéticos. Nenhum dos sistemas de exigências nutricionais avaliados foram eficientes para a predição do desempenho animal. Na raça Nelore, o ganho médio diário observado foi de 1,53 kg/dia, com valores preditos de 1,53; 1,70 e 1,82 kg/dia pelos sistemas NRC, CNCPS e BR-CORTE. Apesar de os valores médio e preditos pelo sistema NRC terem sido semelhantes, de acordo com a equação de regressão, o intercepto e a inclinação foram diferentes de zero e um. Na raça Red Norte, o desempenho observado foi de 1,88 kg/dia com valores preditos de 1,50; 1,66 e 1,72 kg/dia pelos sistemas NRC, CNCPS e BR-CORTE, provavelmente porque o banco de dados desses sistemas é baseado principalmente em resultados obtidos em bovinos Angus.

The objective of this work was to evaluate average daily gain (ADG) and carcass traits in Nellore and Red Norte steers, finished in feedlots and to evaluate performance predictions by using the systems BR-CORTE, CNCPS 5.0 and NRC (2000). It was used 41 steers: 19 Nelore animals with initial body weight of 361 ± 31 kg and 22 Red Norte animals with initial body weight of 367 ± 30 kg. Adaptation period lasted 28 days. Animal performance evaluation was composed of three 28 day period, totaling 84 days. At the end of each period, animals were weighed after a 16-hour feeding fast. Average gain weight of Red Norte steers was greater than Nellore breed animals (1.43 vs. 1.81 kg/day, respectively). Red Norte animals also presented greater loin eye area (75.41 cm² vs. 68.67 cm²). It was not observed any differences on subcutaneous fat thickness and on rump fat among the genetic groups. None of the nutritional requirement system evaluated were efficient in predicting animal performance. For Nellore breed, daily average gain observed was 1.53kg/day, with values of 1.53, 1.70 and 1.82 kg/day predicted by NRC, CNCPS and BR-CORTE systems. Although average values and predicted by NRC were similar, according to the regression equation, intercept and inclination were different from zero and one. For Red Norte breed, performance observed was 1.88 kg/day with values of 1.50, 1.66 and 1.72 predicted by the systems NRC, CNCPS and BR-CORTE, probably because database of those systems is based mainly on results obtained from Angus bovines.

O objetivo neste trabalho foi avaliar a qualidade da carne do músculo longissimus thoracis de bovinos durante a maturação. Amostras de 22 bovinos Nelore e 22 Red Norte machos, com 24 meses de idade, foram coletadas às 24 horas post mortem, mantidas a 2oC e analisadas aos 1, 7, 14 e 21 dias. Os animais foram terminados em confinamento (112 dias) com silagem de milho (50%) e concentrado (50%) à vontade. Os valores de pH final, perda por cocção, umidade, proteína, gordura e cinzas foram semelhantes entre as amostras de animais Nelore e Red Norte. O teor de vermelho (a*) e a intensidade de amarelo (b*) foram semelhantes entre as carnes dos dois grupos genéticos, porém a luminosidade (L*) foi maior nas amostras de animais Red Norte. A maturação afetou significativamente a luminosidade, o teor de vermelho e amarelo, croma (C*), o ângulo de tonalidade (H*) e a percepção subjetiva da cor (ΔE), de forma que as alterações de cor mais importantes ocorreram entre 7 e 14 dias. A força de cisalhamento na carne dos animais Red Norte foi cerca de 0.9 kg inferior às dos animais Nelore. A maturação influenciou a força de cisalhamento ao longo da maturação e determinou reduções de 1,09; 0,21 e 0,56 kg nos períodos de 1 a 7; 7 a 14 e 14 a 21 dias, respectivamente. O índice de fragmentação miofibrilar foi maior na carne dos animais Red Norte e nas amostras maturadas por 21 dias. A carne dos animais Red Norte apresentou maior luminosidade e maciez. A maturação melhora a maciez das carnes, por reduzir a força de cisalhamento, porém modifica a cor, cujas alterações mais importantes acontecem entre 7 e 14 dias. A escolha do tempo de maturação mais adequado para as carnes bovinas depende do atributo a ser valorizado.

The objective in this study was to evaluate meat quality of longissimus thoracisi muscle during ageing. Samples from 22 Nelore bovines and 22 Red Norte males at 24 months of age were collected at 24 hours post mortem, kept at 2ºC and analyzed on days 1, 7, 14 and 21. The animals were finished in confinement (112 days) with corn silage (50%) and concentrate (50%) ad libitum. Values of final pH, cooking loss, moisture, protein, fat and ash were similar among samples of Nelore and Red Norte animals. The red level (a*) and the yellow intensity (b*) were similar among meats of the two genetic groups, however, luminosity (L*) was higher in samples of Red Norte animals. Ageing significantly affected luminosity, level of red and yellow, chroma, tone angle (H*) and subjective perception of the color (ΔE), thus, the most important changes in color occurred from 7 to 14 days. Shear force in the meat of Red Norte animals was approximately 0.9 kg lower than in Nelore animals. Ageing influenced shear force during maturation with a reduction of 1.09 kg, 0.21 kg and 0.56 kg in the periods from 1 to 7, from 7 to 14 and from 14 to 21 days, respectively. The myofibrillar fragmentation index was higher in the meat of Red Norte animals and in samples aged for 21 days. The meat of Red Norte animals showed more tenderness and luminosity. Ageing improves the tenderness of meat because it reduces shear force, however, it changes the color, whose most important changes take place from 7 to 14 days. The choice of the most appropriate ageing time for beef depends on which attribute will be considered.

Este trabalho foi realizado com os objetivos de avaliar o consumo de matéria seca (CMS) e dos nutrientes da dieta em novilhos Nelore e Red Norte terminados em confinamento e comparar os valores observados aos preditos por meio dos sistemas NRC (2000), CNCPS 5.0 e BR-CORTE. Utilizaram-se 41 novilhos, não-castrados, de dois grupos genéticos, sendo 19 Nelore com peso vivo inicial médio de 361 ± 31 kg e 22 Red Norte com peso vivo inicial de 367 ± 30 kg. No início do período de adaptação, com duração de 28 dias, os animais foram pesados após jejum alimentar de 16 horas e tratados contra endo e ecto parasitas. O período experimental teve duração de 56 dias e, além das pesagens nestes períodos, foram realizadas mensurações do consumo individual, utilizando-se os indicadores LIPE, óxido crômico e matéria seca indigestível (MSi). A comparação entre os dados de consumo observados com aqueles preditos pelos sistemas NRC (2000), CNCPS 5.0 e BR-CORTE foi feita por meio do procedimento PROC REG do software SAS. O consumo de matéria seca expresso em kg/dia não apresentou diferenças entre os grupos genéticos (10,66 vs 10,44). Quando expresso em porcentagem do peso vivo (%PV), o consumo foi maior para os animais da raça Nelore (2,55 vs 2,39%, respectivamente) e, quando expresso em g/kg PV0,75, tendeu a ser maior para a raça Nelore (115 vs 109 g/kg PV0,75). Todos os sistemas avaliados subestimam o consumo de matéria seca, entretanto há menores diferenças entre o consumo observado e o predito para animais mestiços.

The objective of this research was to evaluate the dry matter intake (DMI) and nutrient consumption in Nellore and Red Norte steer finished in a feedlot and compare the actual and predicted values by NRC (2000), CNCPS 5.0 and BR-CORTE systems. Forty-one animals, 19 Nellore and 22 Red Norte steers, with initial live weight of 361 ± 31 kg and 367 ± 30 kg, respectively, were used. The experiment lasted 84 days, with 28 days for adaptation and 56 experimental days. The animals were weighed at the beginning and at end of each 28-day period after 16 hours fasting. The dry matter intake was estimated by LIPE, chrome oxide and indigestible dry matter (DMi) markers. There were no differences between Nellore and Red Norte DMI when expressed in kg/day (10.66 vs. 10.44). When intake was expressed in percentage of live weight (% LW), Nellore steer presented higher intake than Red Norte steer (2.55 vs. 2.39%). All the systems evaluated presented a lower predicted intake than the observed intake. However, these differences were smaller for crossbreed animals.